Part:BBa_K2575001:Design

6X UAS-35S minimal promoter Due to repetitive sequences, the part had to be cloned out of pJAB 1504 using primers to add iGEM restriction sites.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 208
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 208
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 208
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 208
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PCR out of vector, not able to be synthesized in 1 unit

Source

Synthetic/minimal promoter from cauliflower mosaic virus design